Automated high-throughput quantification of mitotic spindle positioning from DIC movies of Caenorhabditis embryos.
The mitotic spindle is a microtubule-based structure that elongates to accurately segregate chromosomes during anaphase.Its position within the cell also dictates the future cell cleavage plan, thereby determining daughter cell orientation within a tissue or cell fate adoption for polarized cells.Therefore, the mitotic spindle ensures at the same t